Aloe Vera Juice and Weight Loss That You Can Learn in a easy way

  

Aloe vera, a common medicinal, readily available, cactus-like plant has several medicinal applications in treating many ailments. Aloe vera under the categories cosmetic, medicinal, food, and nutrition.

This plant being used since Rigvedic times in India, being reported for its medicinal  property  such as treating wounds and burns, protection for skin from X-rays, lung cancer, intestinal problems.

Reduces the blood sugar level in diabetic patients and low-density lipoprotein, increase the high- density lipoprotein, improves the immune system as well as to fight the acquired immune deficiency syndrome (AIDS), and allergies.

Aloe vera gained its industrial importance and the value for their products roughly calculated around $125 million and the recent thrust in utilizing herbal  medicines  have  enlightened  its  significance  in  the industrial development.

A report was found that Americans spend 40 billion dollars on Aloe products as functional foods, drinks, and nutritional supplements Aloe vera  juices are widely used as a health drink, soft drink, laxative drink.

Sherbet and along with several components such as lemon juice, electrolyte, soluble fiber, Vitamin B, amino acids and acetaminophen, other vegetables, yogurts, and cucumber.

Due to its multi-application, the Chinese called the plant as Elixir of youth. The intake of Aloe preparations as a whole leaf extract and as an inner fillet gel enhanced the uptake of water/fat-soluble vitamins in the body and it is the only supplement  known for this ability.

The applications  of Aloe in health are enormous and scientific records on its antiacid, antipeptic, gastroprotective, and antiulcer.The emergence of bioinformatics has led the drug discovery and development process much easier.

Also cost effective and the existing high throughput sequencing techniques added its value. In addition, the juice prepared as a daily dietary ingredient, the present study focuses to study the potentials of Aloe vera compounds in interacting the antiobesity target.

The target chosen has significant functionality in regulating cell metabolism  and  survival. The recent studies have found that deletion of adipocyte- specific IP6K1 modulates AMP-activated protein kinase -mediated adipocyte energy metabolism thereby regulates the fat accumulation in the body.

Secondary metabolites of plants have gained momentum in the process of drug discovery. Recently, arteether, galantamine, nitisinone, and tiotropium have been introduced in the US market.Even more, the plant natural compounds were scientifically evaluated its efficiency against several dreadful diseases such as cancer, HIV/ AIDS, Alzheimer’s, and malaria.

MATERIALS AND METHODS

Beverage preparation

The beverage preparation consists of three steps which include juice processing of selected fruits for improving taste, Aloe vera juice processing and beverage preparation.

The three citrus fruits juices of mosambi, orange, and lemon as well as the pineapple and muskmelon were chosen and added with natural sweeteners in the form of powder (stevia and honey).

The Aloe vera leaves were bought from Pallavaram Market, Chennai. The juices of each fruit were prepared in the following methods. The skins of mosambi was removed and roughly blended to extract the juice and stored immediately since citrus fruits have a tendency to turn to bitter taste.

In the case of orange and lemon, squeezing techniques were used to obtain the extract and mixed with 3/4th of water. In pineapple and muskmelon, the skin was removed, and the pulp was diced into pieces to prepare as juice.

The prepared juices were kept aside and/or stored in the refrigerator until the Aloe vera leaves were extracted. Aloe vera leaves were peeled, diced, and washed several times to remove the stickiness and pulp was extracted by the blending method and processed into juice.

Furthermore, it was filtered through a strainer and stored in clean glass jar. As a final step, the beverage was prepared by mixing together the 30 ml of each fruit juices with 30 ml of Aloe vera juice separately. The mixture was blended nicely using cocktail shaker with the addition of sweeteners.

Determination of phytochemicals and nutraceuticals Phytochemical analysis

The phytochemical analysis was carried out for the food samples prepared for the presence of tannins, flavonoids, steroids, alkaloids, and polyphenols.

The samples were extracted with hydroalcoholic solvents (70%) (70 ml ethanol and 30 ml of water) for 24 h, and the filtrates were analyzed for phytochemicals.

Test for tannins

About 1 ml of the sample was boiled in 20 ml of water in a test tube and then filtered. A few drops of 0.1% ferric chloride was added and observed for brownish green or a blue-black coloration.

Test for flavonoids

About 5 ml of dilute ammonia solution were added to a portion of the aqueous filtrate of each plant extract followed by addition of concentrated H S0 . A yellow coloration observed in each extract indicated the presence.

Test for steroids

About 2 ml of acetic anhydride was added to 1 ml of an extract of each sample with 2 ml H2SO4. The color changed from violet to blue or green in some samples indicate the presence of steroids.

Test for alkaloids

Mayer’s test - to a few (1) ml of the extract, a drop of Mayer’s reagent was added by the side of the test tube. A creamy or white precipitate indicates positive.

Test for polyphenols

Ethanol of 4 ml was added to each extract (1 ml) and the resulting solution was transferred in test tubes and kept warm in a water bath for 15 min. Three drops of freshly prepared ferric cyanide solution were added to the extract solution. Formation of a blue-green color indicated the presence of polyphenols.

Determination of weight loss

The efficiency of weight loss was tested using pancreatic lipase inhibitory activity. The lipase enzyme was extracted from the pancreas of chicken (Gallus domesticus).