How Much Do You Know about Green Tea The Benefits Of Health?

  

Green Tea the Benefits of Health

Over the past 15–20 years, a number of other research studies have been conducted to determine what health benefits can be attributed to consumption of green tea and its extracts. This research has shown that green tea has a variety of potential health benefits. These benefits include anticarcinogenic, anti-inflammatory, antimicrobial, and antioxidant properties, and benefits in cardiovascular disease and oral health.

While much of this research has been performed in vitro, and a significant amount of the research done in vivo, using animal models, this will focus mainly on studies conducted with human subjects plus pertinent information from the other types of studies.

 Anticarcinogenic Properties of Green Tea

Cancer is currently a major source of morbidity and mortality worldwide. Billions of dollars in research monies have been poured into cancer research over the past 50 plus years, and yet we do not seem to be any closer to actually curing it. In addition, quite often the chemotherapies do as much, if not more damage to the patient as the disease.

Because cancer appears in so many different forms in multiple parts of the body, it has been difficult to determine the mechanisms that lead to the disease. Even with what we now know about cancer risk factors, there are still many people who seemingly have none of the risk factors, and yet succumb to a rapidly aggressive form. Encouraging people to think about how a healthy lifestyle can prevent disease is certainly a step in the right direction, and it would be most helpful to identify substances that could be useful in prevention and treatment.

The main component of green tea that has been studied in cancer research is EGCG. There are several cancer related mechanisms attributed to EGCG. These include: inhibition of angiogenesis, DNA hypermethylation, NF- B, telomerase activity, and tumor cell proliferation and metastasis; induction of tumor suppressor genes; and promotion of tumor cell apoptosis.

Inhibition of angiogenesis is suggested to occur through a decrease in RNA and peptide levels of vascular endothelial growth factor (VEGF), and by disrupting the dimerization of VEGF with the vascular endothelial growth factor receptor. Another suggested way in which green tea catechins may generally inhibit carcinogenesis is through increasing levels of glutathione S-transferase pi (GST-pi), which catalyzes detoxification reactions that inhibit carcinogen-induced DNA damage.

Analysis of studies performed using human oral consumption of green tea to assess cancer risk showed that case-control studies gave the most consistent results and was positive for reduced cancer risk in breast, cardiac, colorectal, esophageal, gastric, lung, ovarian, pancreatic, and prostate cancers. A recent large study showed a relationship between breast cancer risk and tea consumption, with the risk being highest in the groups that did not consume tea and lowest in the groups that consumed the most cups per day.

Number of cups were assessed in five categories (0.1–1.0 cups, 1.1–2.0 cups, 2.1–3.0 cups, 3.1–5.0 cups, >5.0 cups). Analysis of the types of green tea beverage or extracts used in studies suggests that green tea beverage or a supplement containing mixed catechins may be more effective than using single catechin (e.g., EGCG) supplements.

The potential molecular mechanisms and targets that might explain how green tea catechins possess anticarcinogenic properties have been widely studied (using various cell cultures, etc.),especially in breast cancers. These include interaction with specific proteins, anti-angiogenesis mechanisms, targets for inhibition of enzyme activities and cell signaling pathways, and induction of cell cycle arrest and apoptosis.

The mushrooming area of nanotechnology has lead to the development of potential chemotherapy involving nanoparticles (NPs). Various particles (e.g., gold) can be used to deliver compounds to specific areas of the body. Research using EGCG and nanoparticles has already begun using a number of delivery approaches.

These include: coating an NP, such as gold, with EGCG; use of encapsulated (in liposomes or polymeric NPs) EGCG in NPs along with anti-cancer drugs, outer ligands that will bind to specific targets, or outer polymers that will enhance the intestinal absorption of EGCG.

Cardiovascular Disease Health Benefits

Cardiovascular disease (CVD) is a complex disorder involving multiple factors. Among those factors are inflammation, oxidative stress, platelet aggregation, and lipid metabolism. Some of these factors are also involved in other disease processes, but will be discussed in this paper under CVD.

There have been a number of studies over the years assessing green tea consumption in respect to CVD risk. Two studies from Japan that included nearly 50,000 people found a decreased mortality rate due to CVD based on consumption of various numbers of cups per day. One study showed a 28% decrease in CVD death between those who consumed 3 cups and those who consumed 10 cups.

The other study showed a 14% decrease in CVD mortality between those who consumed <1 cup and those who consumed 5 cups. Other studies in Japan using a green tea extract found that, after 12 weeks, the subjects had reductions in body fat (10%), blood pressure (6.5%), and low-density lipoprotein (LDL) levels (2.6%), suggesting reduced risk of CVD.

After two months, diabetic patients also had reduced fasting blood glucose levels (from 135 to 128.8 mg/dL), and hemoglobin A1c (HBA1c) levels (from 6.2% to 6.0%). A large meta-analysis of 17 studies from over 30 years, including data from Europe, the UK, and the U.S., found that increasing consumption of green tea by three cups per day decreased the risk of myocardial infarction (MI) death by 11%.

Another study showed a decreased risk of mortality in patients who had an acute MI and a history of regular green tea consumption for at least a year prior to the MI. Participants who did not drink green tea had a 14% rate of death due to the MI; participants who drank up to 14 cups per week had an 11% rate of MI death; and participants who drank more than 14 cups per week had a 10% rate of MI death.

An interesting study in patients with CVD showed that consumption of EGCG resulted in a rapid improvement of vascular endothelial function. Participants who ingested an initial dose of 300 mg of EGCG had an improved brachial artery flow-mediated dilation from 7.1% to 8.6% after 2 h. Another recent study found that increased intake of dietary flavonoids was associated with a decreased risk of CVD.

The participants were divided into three groups based on average daily consumption of flavonoids. The first tertile consumed 89 mg/day, the second tertile consumed 251 mg/day, and the third tertile consumed 532 mg/day. The number of deaths due to CVD in the first tertile was 8.6%; in the second tertile, 6.4%; and in the third tertile, 5.0%.

Inflammation

Besides CVD, inflammation is also involved in arthritis, aging, cancer, etc. Many of the anti-inflammatory effects when using green tea have been studied in rheumatoid arthritis (RA) and osteoarthritis (OA), and are pertinent to CVD as well. Some general anti-inflammatory mechanisms of green tea components are:

increased production of the anti-inflammatory cytokine, IL-10; regulation of IL-6 synthesis and signaling; decreased production of destructive matrix metalloproteinase’s via TNF-induced phosphorylation of mitogen-activated protein kinases (MAPKs); and decreased expression of the chemokine receptor CCR2 and decreased levels of the proinflammatory cytokines IL-1 and TNF-.

The specific studies on inflammation can be roughly categorized into: inhibition of neutrophil-endothelium interaction, modulation of neutrophil functions and death, and regulation of inflammation factors. Neutrophil migration and function is an integral part of the inflammatory response, so controlling neutrophils is vital in decreasing inflammation.

Studies have shown that green tea catechins cause a reduction in the number of leukocyte-endothelial cell adhesion molecules (CAMs), such as ICAM-1, VCAM-1, and E-selection, expressed on the endothelial cell surface. This restricts the ability of the neutrophils to migrate to sites of infection.

Other studies have shown that factors known to regulate neutrophil function. such as IL-1 , IL-2, TNF- , and granulocyte-macrophage colony-stimulating factor (GM-CSF), are suppressed by consumption of green tea or EGCG, resulting in inhibition of inflammation. Studies on the inhibition of pro-inflammatory factors have shown that green tea catechins downregulate many inflammatory.

Oxidative Stress

Oxidative stress in the body is closely tied to inflammation and CVD, and is the result of the damaging effects of reactive oxygen species (ROS). These ROS are capable of causing chronic inflammation through induction of inflammatory cytokines and chemokines, and pro-inflammatory transcription factors.

In general, green tea catechins have been found to have antioxidant activity through: inhibiting redox sensitive transcription factors and pro-oxidant enzymes, scavenging ROS, and inducing anti-oxidant enzymes. Studies to determine the antioxidant capabilities of green tea may measure a variety of substances.

Tests may measure the presence of known ROS or their metabolites, such as hydroxyl radical, peroxides, superoxide, and singlet oxygen. Other measurements may be for known antioxidant substances such as superoxide dismutase (SOD) and glutathione peroxidase, or substances that indicate inflammation such as high-sensitivity C-reactive protein (hs-CRP) and TNF.

Another type of testing assesses total antioxidant capacity (TAC), also known as total antioxidant status (TAS), which measures the amount of oxidants that are neutralized in the body (e.g., moles of oxidant neutralized by 1 L of plasma), with a lower number translating into a higher risk of disease .

The results from recent studies have shown that green tea catechins can affect levels of ROS , increase levels of antioxidants  , decrease levels of inflammatory substances , and increase TAC (TAS). An excellent summary of earlier studies that measured ROS and TAC can be found in a chapter by Serafini et al. 2011.

Platelet Aggregation

Platelet activation and subsequent aggregation play an important role in CVD. When the vascular endothelium is damaged, platelets usually respond rapidly and aggregate to form plugs at the damage site, and may also form clots that could lead to vessel occlusion. Many of the studies on platelet aggregation have been carried out using various animal platelets.

In addition to showing that green tea catechins were involved in inhibition of platelet aggregation, studies suggested that catechins may affect several cellular targets that are related to platelet activation, including: through the arachadonic acid pathway, inhibition of a cytoplasmic increase in calcium, decreased thrombaxane A₂ (TXA₂) production, and inhibition of cyclooxygenase-1 (COX)-1.

A study using human platelets concluded that EGCG was able to inhibit platelet activation by adenosine diphosphate (ADP) stimulation, and suppressed the p38 MAPK phosphorylation of heat shock protein 27 (HSP27), which would inhibit the release of pro-thrombotic contents from platelets.

Lipid Metabolism

Increased blood lipid levels have long been suspected as an increased risk for CVD, especially LDLs. One mechanism that is linked with atherosclerosis is the presence of oxidated LDL . There have been many studies performed using humans subjects to determine the effect of green tea catechins on lipids. The studies have reported that consumption of green tea catechins lowers total cholesterol and LDL levels and also reduces blood pressure.

In addition, a recent study found that green tea catechins are incorporated into LDL particles, and are then able to reduce the oxidation of LDL. Catechins prevent LDL oxidation via radical-trapping abilities and act as hydrogen donors to -tocopherol radicals.

Oral Health Benefits

During the course of the many research studies done using green tea catechin consumption, it was noticed that the research subjects seemed to have improved oral health after consumption. Research was then launched to focus on the effects of green tea on oral health.

Two of the general ways in which green tea consumption helps oral health are due to its anti-inflammatory properties, and antimicrobial activity against mouth flora such as Streptococcus mutans . The antimicrobial activity may also be responsible for the improvement observed as to bad breath the two major types of effects on oral health are a decrease in periodontist and dental caries.

 Periodontitis

Green tea consumption has been found to result in decreased tooth loss, and prevent the development and progression of periodontitis. Green tea consumption also has positive effects on periodontal health when assessed as to probing depth, attachment loss, gingival bleeding, and dentin erosion.

In addition to the antimicrobial effects on the main bacteria involved in gingivitis, Porphyromonas gingival is, EGCG has been shown to inhibit the ability of the bacteria to bind to oral epithelial cells via fimbriae, and has also been shown to inactivate bacterial collagenases. EGCG also inhibits production of matrix metalloproteins and IL-8, which are responsible for initiating tissue destruction.

Dental Caries

Prevention of dental caries is attributed to the ability of EGCG to bind and inhibit salivary and bacterial amylases, in particular, -amylase. EGCG also prevents generation of acid from carbohydrates through inhibiting the transcription and function of LDH.

One of the main things that encourages tooth decay is that the major oral bacteria (e.g., Streptococcus mutans) form a biofilm on the surface of teeth. EGCG inhibits the adherence of the bacteria to teeth, decreases biofilm formation, and inhibits the ability of the bacteria to produce an acid environment. EGCG also inhibits the hydrogen binding and hydrophobic interactions of bacterial collagenases. Consumption of green tea has also been associated with an increase in oral peroxidase activity.

Conclusions

Green tea catechins have proved to be very versatile in providing health benefits. This means that there are potential health benefits for everyone in the consumption of green tea. Even moderate amounts of consumption (drinking 1–2 cups of tea per day) may have benefits. It is a very good thing that it is the second most popular beverage worldwide, as the differences in health in a world without green tea might be significant.

There is fortunately a wide variety of research being performed using green tea catechins, and we are starting to see many studies performed using human subjects, as it is extremely important that we are able to show the direct benefits to humans. The expansive repertoire of green tea activity in health is important, especially to those people who live where medical assistance is not generally available or affordable.